Liquefaction of Semen Generates and Later Degrades a Conserved 1 Semenogelin Peptide that Enhances HIV Infection 2 3
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چکیده
Semenogelin Peptide that Enhances HIV Infection 2 3 Nadia R. Roan , Haichuan Liu, Shariq M. Usmani, Jason Neidleman, Janis A. 4 Müller, Aram Avila-Herrera, Ali Gawanbacht, Onofrio Zirafi, Simon Chu, Ming Dong, 5 Senthil T. Kumar, James F. Smith, Katherine S. Pollard , Marcus Fändrich, Frank 6 Kirchhoff, Jan Münch, H. Ewa Witkowska, and Warner C. Greene 9 7 8 9 1 Gladstone Institute of Virology and Immunology, 2 Department of Urology, 3 Department 10
منابع مشابه
Liquefaction of semen generates and later degrades a conserved semenogelin peptide that enhances HIV infection.
UNLABELLED Semen enhances HIV infection in vitro, but how long it retains this activity has not been carefully examined. Immediately postejaculation, semen exists as a semisolid coagulum, which then converts to a more liquid form in a process termed liquefaction. We demonstrate that early during liquefaction, semen exhibits maximal HIV-enhancing activity that gradually declines upon further inc...
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During liquefaction of the ejaculate, the semen coagulum proteins semenogelin I (SEMG1) and semenogelin II (SEMG2) are degraded to low molecular mass fragments by kallikrein-related peptidase 3 (KLK3), also known as prostate-specific antigen. Semenogelin molecules initiate their own destruction by chelating Zn(2+) that normally would completely inhibit the proteolytic activity of KLK3. In a sim...
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Semen liquefaction changes semen from a gel-like to watery consistency and is required for sperm to gain mobility and swim to the fertilization site in the Fallopian tubes. Kallikrein-related peptidases 3 (KLK3) and other kallikrein-related peptidases from male prostate glands are responsible for semen liquefaction by cleaving gel-forming proteins (semenogelin and collagen). In a physiological ...
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SEM1(86-107) is a 22-residue peptide corresponding to residues 86-107 in the semenogelin I protein. SEM1(86-107) is an abundant component of freshly liquefied semen and forms amyloid fibrils capable of enhancing HIV infection. To probe the factors affecting fibril formation and gain a better understanding of how differences in pH between semen and vaginal fluid affect fibril stability, this stu...
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In the present study we demonstrate that both X4- and R5-tropic HIV-1 strains are able to infect the human epithelial cell line HT-29. Infection was enhanced 2-fold when HIV was added to semen before contact with the cell cultures. The enhancing effect of semen was complement dependent, as evidenced by blockage of generation of C3a/C3a(desArg) in semen by heat or EDTA treatment of semen and sup...
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تاریخ انتشار 2014